The Study of Microbial Structure: Microscopy and Specimen Preparation contd...
2. Dark Field Microscope
In the dark field microscope the background remains dark & specimen is illuminated. It contains a special condenser lens mounted under the stage which don't permit light to be transmitted directly to specimen & into objective lens. The dark field condenser lens focus light on the specimen at an oblique angle such that light that don't reflect of an object doesn't enter the objective lens. Therefore, only light that reflects off specimen will be seen & the light simply passing via slide will not enter the objective lens. So the microorganism bright on the dark background. Dark field microscope diagnosis of syphilis by detecting the causative agent Treponema palladium which don't stain well. It is also important to study motility life of procaryotic & eucaryotic cells.
In the dark field microscope the background remains dark & specimen is illuminated. It contains a special condenser lens mounted under the stage which don't permit light to be transmitted directly to specimen & into objective lens. The dark field condenser lens focus light on the specimen at an oblique angle such that light that don't reflect of an object doesn't enter the objective lens. Therefore, only light that reflects off specimen will be seen & the light simply passing via slide will not enter the objective lens. So the microorganism bright on the dark background. Dark field microscope diagnosis of syphilis by detecting the causative agent Treponema palladium which don't stain well. It is also important to study motility life of procaryotic & eucaryotic cells.
fig: Dark field microscope
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