ASPECTS OF MOLECULAR BIOLOGY & BIOINFORMATICS RELEVANCE IN INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY CONTD..
The Polymerase Chain Reaction (PCR) is a technology used to amplify small amounts of
DNA. The PCR technique was invented in 1985 by Kary B. Mullis while working as a
chemist at the Cetus Corporation, a biotechnology firm in Emeryville, California. So
useful is this technology that Mullis won the Nobel Prize for its discovery in 1993, eight
years later. It has found extensive use in a wide range of situations, from the medical
diagnosis to microbial systematics and from courts of law to the study of animal
The requirements for PCR are:
a. The DNA or RNA to be amplified
b. Two primers
c. The four nucleotides found in the nucleic acid,
d. A heat stable a thermostable DNA polymerase derived from the thermophilic
bacterium, Thermus aquaticus, Taq polymerase
The Primer: A primer is a short segment of nucleotides which is complementary to a
section of the DNA which is to be amplified in the PCR reaction.
Primers are anneal to the denatured DNA template to provide an i…